Molecular Gating of an Engineered Enzyme Captured in Real Time.
Kokkonen P, Sykora J, Prokop Z, Ghose A, Bednar D, Amaro M, Beerens K, Bidmanova S, Slanska M, Brezovsky J, Damborsky J, Hof M.
Kokkonen P, et al.
J Am Chem Soc. 2018 Dec 26;140(51):17999-18008. doi: 10.1021/jacs.8b09848. Epub 2018 Dec 14.
J Am Chem Soc. 2018.
PMID: 30501200
Photoinduced electron-transfer-fluorescence correlation spectroscopy (PET-FCS) has enabled real-time observation of molecular gating at the single-molecule level with rate constants ( k(on) = 1822 s(-1), k(off) = 60 s(-1)) corresponding well with those from the pre-steady- …
Photoinduced electron-transfer-fluorescence correlation spectroscopy (PET-FCS) has enabled real-time observation of molecular gating at the …